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Image Search Results
Journal: bioRxiv
Article Title: Novel small RNAs expressed by Bartonella bacilliformis under multiple conditions reveal potential mechanisms for persistence in the sand fly vector and human host
doi: 10.1101/2020.08.04.235903
Figure Lengend Snippet: Eight separate Northern blots were run under identical experimental conditions (see Materials and methods). RNA ladders from the respective blots were aligned with each other for presentation of the resolved total RNA. sRNA designations are shown above each blot. Exposure times (minutes, m; seconds, s) and origin of the RNA are indicated below each blot.
Article Snippet: qRT-PCR was done on cDNA synthesized from 16 ng
Techniques: Northern Blot
Journal: bioRxiv
Article Title: Novel small RNAs expressed by Bartonella bacilliformis under multiple conditions reveal potential mechanisms for persistence in the sand fly vector and human host
doi: 10.1101/2020.08.04.235903
Figure Lengend Snippet: A) PCR analysis of an in vitro -transcribed (IVT) region of B. bacilliformis (Bb) genomic DNA (gDNA) containing BbgpI. Ethidium bromide-stained agarose (1%) gels are shown. PCR on the resulting cDNA using “Nested Primers” (see S4A Fig) produced unspliced DNA (450-bp band), a partially-spliced product of ~390 bp, plus a 218-bp band corresponding to the BbgpI flanking region where BbgpI self-spliced out (indicated by the red arrow). PCR on a Bb IVT RNA negative control did not produce product. B) As in A) but utilizing cDNA synthesized from B. bacilliformis total RNA using “Splice Flank Primers” (see S4A Fig) . A 308-bp amplicon was produced from gDNA, whereas a 76-bp band, corresponding to the BbgpI flanking region with BbgpI spliced out, was produced from cDNA generated from total RNA (indicated by the red arrow). PCR on a Bb total RNA negative control did not produce product.
Article Snippet: qRT-PCR was done on cDNA synthesized from 16 ng
Techniques: In Vitro, Staining, Produced, Negative Control, Synthesized, Amplification, Generated